from PIL import Image
from PIL.ExifTags import TAGS
import os

PATH=os.getcwd()

i = Image.open(PATH+'\\1_horses.jpg')
os.remove(PATH+'\\1_horses.jpg')

Traceback (most recent call last):
  File "D:\Ильнур\Мои документы\Desktop\фото\1.py", line 8, in <module>
    os.remove(PATH+'\\1_horses.jpg')
WindowsError: [Error 32] Процесс не может получить доступ к файлу,: 'D:\\\xc8\xeb\xfc\xed\xf3\xf0\\\xcc\xee\xe8 \xe4\xee\xea\xf3\xec\xe5\xed\xf2\xfb\\Desktop\\\xf4\xee\xf2\xee\\1_horses.jpg'

from PIL import Image
from PIL.ExifTags import TAGS
import os

PATH=os.getcwd()

i = Image.open(PATH+'\\1_horses.jpg')
del(i)
os.remove(PATH+'\\1_horses.jpg')

fp = open(filename, "rb")
im = Image.open(fp) # open from file object
im.load() # make sure PIL has read the data
fp.close()