for text in file.readlines():
        text = text.rstrip()
        regex = re.findall(';[0-2]?[0-9][0-9]?.[0-2]?[0-9][0-9]?.[0-2]?[0-9][0-9]?.[0-2]?[0-9][0-9]?;', text)
        if regex is not None and regex not in ips:
            ips.append(regex)

    regex_ = re.compile(';[0-2]?[0-9][0-9]?.[0-2]?[0-9][0-9]?.[0-2]?[0-9][0-9]?.[0-2]?[0-9][0-9]?;')
    for text in file.readlines():
        text = text.rstrip()
        regex = re.findall(regex_, text)
        if regex is not None and regex not in ips:
            ips.append(regex)
    print(time.time()-ts)

if regex is not None and regex not in ips:

if not regex in ips:

l = range(10000)
s = set(range(10000))
def list_test():
	return 5088 in l
def set_test():
	return 5088 in s
if __name__ == '__main__':
	import timeit
	print(timeit.timeit("list_test()", setup="from __main__ import l, list_test", number=1000))
	print(timeit.timeit("set_test()", setup="from __main__ import s, set_test", number=1000 ))

0.0621750354767
0.000143051147461

tuple(text.rstrip().split(";"))

regex not in ips:
            ips.append(regex)

text = text.rstrip()

ips = {}
for line in f.readlines():
    ips[line.split(';')[-2]] = None
ips = ips.keys()