class Ui_MainWindow(object):
     def setupUi(self, MainWindow):
          #код, преобразующий переданное MainWindow
     def retranslateUi(self, MainWindow):
          #код, создающий русские надписи

#Разные важные import
from Designes.form1 import Ui_MainWindow
class mainForm(Ui_MainWindow):
#Инициализация
     def __init__(self):
         Ui_MainWindow.__init__(self)
         self.window = QtWidgets.QMainWindow() #окно, которое должно быть передано 
         self.setupUi(self.window) #этой функции и преображено ей
         self.sett.clicked.connect(self.sett_onClick) #назначаем свои обработчики событий элементам
#..................
        self.window.show()
     def sett_onClick(self):
          #код обработки события клика по кнопке "настройки"
#...................
if __name__ == "__main__":
     app = QtWidgets.QApplication(sys.argv)
     form = maneForm()
     sys.exit(app.exec_())

self.btnExit.clicked.connect(MainWindow.close)

<?xml version="1.0" encoding="UTF-8"?>
<ui version="4.0">
 <class>MainWindow</class>
 <widget class="QMainWindow" name="MainWindow">
  <property name="enabled">
   <bool>true</bool>
  </property>
  <property name="geometry">
   <rect>
    <x>0</x>
    <y>0</y>
    <width>800</width>
    <height>600</height>
   </rect>
  </property>
  <property name="windowTitle">
   <string>QMainWindow</string>
  </property>
  <property name="statusTip">
   <string/>
  </property>
  <widget class="QWidget" name="centralwidget">
   <property name="statusTip">
    <string/>
   </property>
  </widget>
  <widget class="QMenuBar" name="menubar">
   <property name="geometry">
    <rect>
     <x>0</x>
     <y>0</y>
     <width>800</width>
     <height>21</height>
    </rect>
   </property>
   <widget class="QMenu" name="menuFile">
    <property name="statusTip">
     <string extracomment="0"/>
    </property>
    <property name="accessibleDescription">
     <string/>
    </property>
    <property name="title">
     <string>&amp;File</string>
    </property>
    <addaction name="action_Exit"/>
   </widget>
   <addaction name="menuFile"/>
  </widget>
  <widget class="QStatusBar" name="statusbar"/>
  <action name="action_Exit">
   <property name="text">
    <string>Exit</string>
   </property>
   <property name="statusTip">
    <string>exit?</string>
   </property>
  </action>
 </widget>
 <resources/>
 <connections>
  <connection>
   <sender>action_Exit</sender>
   <signal>triggered()</signal>
   <receiver>MainWindow</receiver>
   <slot>close()</slot>
   <hints>
    <hint type="sourcelabel">
     <x>-1</x>
     <y>-1</y>
    </hint>
    <hint type="destinationlabel">
     <x>399</x>
     <y>299</y>
    </hint>
   </hints>
  </connection>
 </connections>
</ui>

[guest@localhost mainwindow]$ pyuic4 mainwindow.ui
# -*- coding: utf-8 -*-

# Form implementation generated from reading ui file 'mainwindow.ui'
#
# Created: Thu Nov 27 20:00:20 2014
#      by: PyQt4 UI code generator 4.10.2
#
# WARNING! All changes made in this file will be lost!

from PyQt4 import QtCore, QtGui

try:
    _fromUtf8 = QtCore.QString.fromUtf8
except AttributeError:
    def _fromUtf8(s):
        return s

try:
    _encoding = QtGui.QApplication.UnicodeUTF8
    def _translate(context, text, disambig):
        return QtGui.QApplication.translate(context, text, disambig, _encoding)
except AttributeError:
    def _translate(context, text, disambig):
        return QtGui.QApplication.translate(context, text, disambig)

class Ui_MainWindow(object):
    def setupUi(self, MainWindow):
        MainWindow.setObjectName(_fromUtf8("MainWindow"))
        MainWindow.setEnabled(True)
        MainWindow.resize(800, 600)
        MainWindow.setStatusTip(_fromUtf8(""))
        self.centralwidget = QtGui.QWidget(MainWindow)
        self.centralwidget.setStatusTip(_fromUtf8(""))
        self.centralwidget.setObjectName(_fromUtf8("centralwidget"))
        MainWindow.setCentralWidget(self.centralwidget)
        self.menubar = QtGui.QMenuBar(MainWindow)
        self.menubar.setGeometry(QtCore.QRect(0, 0, 800, 21))
        self.menubar.setObjectName(_fromUtf8("menubar"))
        self.menuFile = QtGui.QMenu(self.menubar)
        self.menuFile.setStatusTip(_fromUtf8(""))
        self.menuFile.setAccessibleDescription(_fromUtf8(""))
        self.menuFile.setObjectName(_fromUtf8("menuFile"))
        MainWindow.setMenuBar(self.menubar)
        self.statusbar = QtGui.QStatusBar(MainWindow)
        self.statusbar.setObjectName(_fromUtf8("statusbar"))
        MainWindow.setStatusBar(self.statusbar)
        self.action_Exit = QtGui.QAction(MainWindow)
        self.action_Exit.setObjectName(_fromUtf8("action_Exit"))
        self.menuFile.addAction(self.action_Exit)
        self.menubar.addAction(self.menuFile.menuAction())

        self.retranslateUi(MainWindow)
        QtCore.QObject.connect(self.action_Exit, QtCore.SIGNAL(_fromUtf8("triggered()")), MainWindow.close)
        QtCore.QMetaObject.connectSlotsByName(MainWindow)

    def retranslateUi(self, MainWindow):
        MainWindow.setWindowTitle(_translate("MainWindow", "QMainWindow", None))
        self.menuFile.setTitle(_translate("MainWindow", "&File", None))
        self.action_Exit.setText(_translate("MainWindow", "Exit", None))
        self.action_Exit.setStatusTip(_translate("MainWindow", "exit?", None))

[guest@localhost mainwindow]$

QtCore.QObject.connect(self.action_Exit, QtCore.SIGNAL(_fromUtf8("triggered()")), MainWindow.close)