import csv
fread = csv.reader(open('D:\\Proj\\Gmail\\test.txt'), delimiter='\t')
line1 = fread.next()
line2 = line1.reverse()
print ' '.join(line2)

Traceback (most recent call last):
  File "D:\Proj\Gmail\1.py", line 8, in <module>
    print ' '.join(line2)
TypeError

print line1

['', '134,92', '54,75']

54,75	134,92	

line2 = line1.reverse()

 line1.reverse()

54,75	134,92	
54,81	135,27		
54,61	135,37
54,56	135,01

with open('Путь', 'r') as file_:
    all_line = []
    for line in file_:
        all_line.append(line.strip().split())
print all_line

with open('Путь', 'r') as file_:
    all_line = [line.strip().split() for line in file_]
print all_line

fread = open('D:\\Proj\\Kolya_img\\Gmail\\test.txt')
line1 = fread.readline()[1]
print (line1)

line1 = fread.readline() # Убрать [1]

with open('Путь', 'r') as file_:
    all_line = []
    for line in file_:
        all_line.extend(line.strip().split())
print all_line

with open('Путь', 'r') as file_:
    all_line = [float(digit) for line in file_ for digit in line.strip().split()]
print all_line