условие, конечно же, размытое, вот как-то в этом направлении попробуй
matrix_1 = [
[1, 2, 3],
[4, 5, 6],
[7, 8, 9],
]
matrix_2 = [
[9, 8, 7],
[6, 5, 4],
[3, 2, 1],
]
num_col_m_1 = int(input('enter the column number of the matrix-1 from 1 to {}'.format(len(matrix_1[0]))))-1
num_col_m_2 = int(input('enter the column number of the matrix-2 from 1 to {}'.format(len(matrix_1[0]))))-1
col_m_1 = [val[num_col_m_1] for val in matrix_1]
col_m_2 = [val[num_col_m_2] for val in matrix_2]
print('columns to compare', col_m_1, col_m_2)