myIter = 1
with open("myfile") as f:
    while 1:
        myIter += 1
        f.seek(-myIter, 2)
        sim = f.read(1)
        if sim == u'\n': 
            res = f.readline().rstrip()
            break
    print res

s = ''
cur_block_size = block_size = 1024
with open('users.txt') as f:
    while True:
        f.seek(-1*cur_block_size, 2)
        s = f.read(block_size) + s
        res = s.split('\n')
        if len(res) > 2:
            print res[-2]
            break
        cur_block_size += block_size

from itertools import repeat

def reverse_readline (f_path):
	f = open(f_path, 'r')
	f.seek(0, 2)
	it = ( '' if f.tell() == 1 else (f.seek(-2, 1) or f.read(1)) for i in repeat(None))
	while True: 
		st = ''
		for ch in it:
			if ch == '': raise StopIteration()
			if ch == '\n': break
			else: st = ch + st
		yield st

def read_last(f_path):
	f = open(f_path, 'r')
	f.seek(0, 2)
	it = ( '' if f.tell() == 1 else (f.seek(-2, 1) or f.read(1)) for i in repeat(None))
	while True: 
		st = ''
		for ch in it:
			if ch == '\n' or not ch: break
			else: st = ch + st
		yield st
		if not ch: raise StopIteration()

import os
def LastRow(fileName,MAX_ROW=200):
    """отдать последнюю строку файла"""
    with open(fileName,"rb") as f:
        f.seek(-min(os.path.getsize(fileName),MAX_ROW),2)
        return (f.read().splitlines())[-1]

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