import _winreg
key = _winreg.OpenKey(_winreg.HKEY_CURRENT_USER,"SOFTWARE\\Microsoft\\Windows\\CurrentVersion\\Run",0, _winreg.KEY_ALL_ACCESS)
_winreg.SetValueEx(key, "Strike", 0, _winreg.REG_SZ,"D:\Games\sozdanie puti.py")
key.Close()

HKEY_CURRENT_USER

HKEY_LOCAL_MACHINE

Traceback (most recent call last):
  File "C:\Users\СК\Desktop\reestr.py", line 3, in <module>
    key = _winreg.OpenKey(_winreg.HKEY_LOCAL_MACHINE,"SOFTWARE\\Microsoft\\Windows\\CurrentVersion\\Run",0, _winreg.KEY_ALL_ACCESS)
WindowsError: [Error 5] 

(_winreg.KEY_WOW64_64KEY + _winreg.KEY_ALL_ACCESS)

 _winreg.KEY_ALL_ACCESS

Traceback (most recent call last):
  File "C:\Users\СК\Desktop\reestr.py", line 5, in <module>
    _winreg.SetValueEx(key, "Strike", 0, _winreg.REG_SZ,"D:\Games\sozdanie puti.py")
WindowsError: [Error 5]