import pytube
link = "https://www.youtube.com/watch?v=WsJ7lE8eRPQ"
itag = 43
url = pytube.YouTube(link).streams.get_by_itag(itag).url
process_call_str = 'ffmpeg -ss {1} -to {2} -i "{0}"'\
                   '-acodec aac -b:a 192k -avoid_negative_ts make_zero "{3}"'
                            format(str(url), str("00:10:00.00"), str("00:10:56.00"), "C:\Users\Anton\YandexDisk\Скриншоты")
status = subprocess.check_call(process_call_str, shell=True)