import pyautogui
button7location = pyautogui.locateOnScreen('calc7key.png')
button7location

 C:\Project\Clicker\venv\Scripts\python.exe C:/Project/Clicker/venv/clicker.py
Traceback (most recent call last):
  File "C:\Project\Clicker\venv\clicker.py", line 2, in <module>
    button7location = pyautogui.locateOnScreen('calc7key.png')
  File "C:\Project\Clicker\venv\lib\site-packages\pyautogui\__init__.py", line 175, in wrapper
    return wrappedFunction(*args, **kwargs)
  File "C:\Project\Clicker\venv\lib\site-packages\pyautogui\__init__.py", line 213, in locateOnScreen
    return pyscreeze.locateOnScreen(*args, **kwargs)
  File "C:\Project\Clicker\venv\lib\site-packages\pyscreeze\__init__.py", line 359, in locateOnScreen
    screenshotIm = screenshot(region=None) # the locateAll() function must handle cropping to return accurate coordinates, so don't pass a region here.
  File "C:\Project\Clicker\venv\lib\site-packages\pyscreeze\__init__.py", line 134, in wrapper
    raise PyScreezeException('The Pillow package is required to use this function.')
pyscreeze.PyScreezeException: The Pillow package is required to use this function.
Process finished with exit code 1

 import pyautogui
button7location = pyautogui.locateOnScreen('calc7key.png', confidence=0.5)
button7location

 C:\Project\Clicker\venv\Scripts\python.exe C:/Project/Clicker/venv/clicker.py
Traceback (most recent call last):
  File "C:\Project\Clicker\venv\lib\site-packages\numpy\__init__.py", line 305, in <module>
    _win_os_check()
  File "C:\Project\Clicker\venv\lib\site-packages\numpy\__init__.py", line 302, in _win_os_check
    raise RuntimeError(msg.format(__file__)) from None
RuntimeError: The current Numpy installation ('C:\\Project\\Clicker\\venv\\lib\\site-packages\\numpy\\__init__.py') fails to pass a sanity check due to a bug in the windows runtime. See this issue for more information: https://tinyurl.com/y3dm3h86
Traceback (most recent call last):
  File "C:\Project\Clicker\venv\clicker.py", line 2, in <module>
    button7location = pyautogui.locateOnScreen('calc7key.png', confidence=0.5)
  File "C:\Project\Clicker\venv\lib\site-packages\pyautogui\__init__.py", line 175, in wrapper
    return wrappedFunction(*args, **kwargs)
  File "C:\Project\Clicker\venv\lib\site-packages\pyautogui\__init__.py", line 213, in locateOnScreen
    return pyscreeze.locateOnScreen(*args, **kwargs)
  File "C:\Project\Clicker\venv\lib\site-packages\pyscreeze\__init__.py", line 360, in locateOnScreen
    retVal = locate(image, screenshotIm, **kwargs)
  File "C:\Project\Clicker\venv\lib\site-packages\pyscreeze\__init__.py", line 340, in locate
    points = tuple(locateAll(needleImage, haystackImage, **kwargs))
  File "C:\Project\Clicker\venv\lib\site-packages\pyscreeze\__init__.py", line 135, in wrapper
    return wrappedFunction(*args, **kwargs)
TypeError: _locateAll_python() got an unexpected keyword argument 'confidence'
Process finished with exit code 1

pip uninstall numpy
pip install numpy==1.19.3