sed -i 's/\r$//' /media/sf_soft/vsc/2.txt

 cmd = str(subprocess.check_output(['/usr/bin/sed -in "s/\r$//" /media/sf_soft/vsc/2.txt'], text=True))

 FileNotFoundError: [Errno 2] No such file or directory: 'sed -in "s/\r$//" /media/sf_soft/vsc/2.txt'

  
subprocess.check_output(['sed', '-in', 's/\r$//', '/media/sf_soft/vsc/2.txt'])

 subprocess.check_output(['sed', '-in', 's/\r$//', '/media/sf_soft/vsc/2.txt'])

 from subprocess import Popen
import subprocess
    command='''
    sed -i 's/\r$//' /media/sf_soft/vsc/2.txt
    '''
    process=Popen(command,shell=True,stdout=subprocess.PIPE)
    result=process.communicate()